Identification of a Tankyrase-binding Motif Shared by IRAP, TAB182, and Human TRF1 but Not Mouse TRF1
نویسندگان
چکیده
منابع مشابه
A shared docking motif in TRF1 and TRF2 used for differential recruitment of telomeric proteins.
Mammalian telomeres are protected by a six-protein complex: shelterin. Shelterin contains two closely related proteins (TRF1 and TRF2), which recruit various proteins to telomeres. We dissect the interactions of TRF1 and TRF2 with their shared binding partner (TIN2) and other shelterin accessory factors. TRF1 recognizes TIN2 using a conserved molecular surface in its TRF homology (TRFH) domain....
متن کاملControl of human telomere length by TRF1 and TRF2.
Telomere length in human cells is controlled by a homeostasis mechanism that involves telomerase and the negative regulator of telomere length, TRF1 (TTAGGG repeat binding factor 1). Here we report that TRF2, a TRF1-related protein previously implicated in protection of chromosome ends, is a second negative regulator of telomere length. Overexpression of TRF2 results in the progressive shorteni...
متن کاملA yeast two-hybrid screen using human TRF1 as bait identified tankyrase, a 142 kDa protein with homology to ankyrins and to the catalytic domain of poly(ADP-ribose)
Telomeres function to ensure the complete replication of chromosome ends, a task which can not be accomplished by conventional DNA polymerases. Telomeres are maintained by telomerase, a reverse transcriptase that adds TTAGGG repeats onto the 3′ ends of vertebrate chromosomes (Greider and Blackburn, 1985; reviewed by Nugent and Lundblad, 1998). Cells have mechanisms to monitor telomere length an...
متن کاملGene therapy with the TRF1 telomere gene rescues decreased TRF1 levels with aging and prolongs mouse health span
The shelterin complex protects telomeres by preventing them from being degraded and recognized as double-strand DNA breaks. TRF1 is an essential component of shelterin, with important roles in telomere protection and telomere replication. We previously showed that TRF1 deficiency in the context of different mouse tissues leads to loss of tissue homeostasis owing to impaired stem cell function. ...
متن کاملTankyrase promotes telomere elongation in human cells
Human telomeres are maintained by telomerase, a reverse transcriptase that adds telomeric repeats to chromosome ends [1,2]. In human tumors and immortalized cells, telomeres are often maintained at a constant length setting [3,4], indicating that telomerase-mediated telomere elongation is tightly regulated. Tankyrase, a telomeric poly(ADP-ribose) polymerase (PARP) [5], was identified through it...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2002
ISSN: 0021-9258
DOI: 10.1074/jbc.m203916200